Difference between revisions of "Part:BBa K864445"

Latest revision as of 17:59, 4 October 2012

Spot42 randomized forward primer

This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system.

This primer is used to create a random sRNA library, together with BBa_K864446, by randomizing the mRNA-binding region of the spot42 small RNA (BBa_K864444). This library can be used to screen for synthetic silencing sRNA:s.

Note

Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 10 N:s, to denote the randomized region, and thus read:

NNNNNNNNNNATTTGGCTGAATATTTTAG

Tm = 51,7°C

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]

12
COMPATIBLE WITH RFC[12]

21
COMPATIBLE WITH RFC[21]

23
COMPATIBLE WITH RFC[23]

25
COMPATIBLE WITH RFC[25]

1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K864445 short</partinfo>
@@ Line 5: / Line 4: @@
 This part is a component of the Uppsala University iGEM 2012 modular small RNA screening system.
-This primer is used to create a random sRNA library, together with <partinfo></partinfo> by randomizing the mRNA-binding region of the spot42 small RNA. The library can be used to screen for synthetic silencing sRNA:s.
+This primer is used to create a random sRNA library, together with <partinfo>K864446</partinfo>, by randomizing the mRNA-binding region of the spot42 small RNA (<partinfo>K864444</partinfo>). This library can be used to screen for synthetic silencing sRNA:s.
+'''Note'''
+Due to technical limitations, the correct sequence can not be entered into the registry. The entered sequence should be preceded by 10 N:s, to denote the randomized region, and thus read:
+NNNNNNNNNN<b>ATTTGGCTGAATATTTTAG
+Tm = 51,7°C
 <!-- Add more about the biology of this part here