Difference between revisions of "Part:BBa K801064:Design"

Latest revision as of 18:30, 3 October 2012

(+)-limonene synthase 1 expression cassette for yeast

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
Illegal AgeI site found at 2320
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 754

This is a composite part consisting of the yeast TEF2 promoter (BBa_K801010), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K122003).
Keywords: limonen, TEF1

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3
Resistance: Cp
Copynumber: high

Protein coding:

BioBrick encodes (+)-limonene synthase
UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]

Enzymatic activity:

E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

BioBrick: BBa_I742111

Organism:

Gene sequence derived from Citrus limon

References

Literature references:

http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002 Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases. Eur J Biochem, 269(13):3160–71.

Database references:

@@ Line 10: / Line 10: @@
 <br>'''Keywords:'''
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
+limonen, TEF1
 <br>'''Abbreviations:'''
@@ Line 39: / Line 39: @@
 '''Backbone:'''<br>
-<!--*Backbone name: pSB1C3'/?backbone_name?-->
+* Backbone name: pSB1C3
-<!--*Resistance: Amp/Cp/Kan/Tet-->
+* Resistance: Cp
-<!--*Copynumber: low/medium/high-->
+* Copynumber: high
 '''Protein coding:'''<br>
-<!--*Protein: ?Name_of_gene_product? [Nucleotide 1 to ???]-->
+* BioBrick encodes (+)-limonene synthase
-<!--*The protein has the amino acid replacements ???99??? to ???99???.-->
+* UniProt entry: [http://www.uniprot.org/uniprot/Q8L5K3 Q8L5K3]
-<!--*The protein encoded is posttranslationally modified by ???.-->
+<!--none/not known/cytotoxic for ''organism name''-->
-<!--*Tag: n-terminally fused/c-terminally fused His5/His6/Strep/Flag/other-->
 '''Enzymatic activity:'''
-<!--none/EC-number ?.?.?.?-->
+* E.C. Number: [http://enzyme.expasy.org/EC/4.2.3.20 4.2.3.20]
 '''Cytotoxicity:'''<br>
@@ Line 69: / Line 68: @@
 '''Source:'''<br>
+*BioBrick: BBa_I742111
 <!--*Commercial system: plasmid name, system name, company name-->
 <!--*Plasmid: p???, provided by ?name_of_person?, ?institute/university?, ?country?-->
@@ Line 79: / Line 79: @@
 '''Organism:'''<br>
+* Gene sequence derived from ''Citrus limon''
 <!--*Genesequence derived from ''?organism_name?''-->
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->
 <!--*Statement about functionality in other chassis.-->
 ===References===
@@ Line 89: / Line 89: @@
 '''Literature references:'''<br>
+*[[http://www.ncbi.nlm.nih.gov/pubmed/12084056 Lücker et al., 2002]] Lücker, J., El Tamer, M. K., Schwab, W., Verstappen, F. W. A., van der Plas, L. H. W., Bouwmeester, H. J., and Verhoeven, H. A. (2002). Monoterpene biosynthesis in lemon (''Citrus limon''). cDNA isolation and functional analysis of four monoterpene synthases. ''Eur J Biochem'', 269(13):3160–71.
 <!--*[http://www.ncbi.nlm.nih.gov/pubmed/?PMID? '''Pubmed:''' ?Author(s)?, ?year?: ?title?]-->