Difference between revisions of "Part:BBa K801064:Design"

Revision as of 15:30, 3 October 2012

(+)-limonene synthase 1 expression cassette for yeast

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 138
Illegal AgeI site found at 2320
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 754

This is a composite part consisting of the yeast TEF2 promoter (BBa_K801010), the Citrus limon (+)-limonene synthase 1 coding region (BBa_K801061) and the yeast TEF1 terminator (BBa_K122003).
Keywords: limonen, TEF1

Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Backbone name: pSB1C3'

Protein coding:

Enzymatic activity: EC-number 4.2.3.20

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

Organism:

Genesequence derived from citrus limon

References

Literature references:

Database references:

@@ Line 10: / Line 10: @@
 <br>'''Keywords:'''
 <!--These keywords are necessary to find your part using a fulltext sarch.-->
-<!--keyword_1, keyword_2, keyword_3, keyword_4, keyword_5-->
+limonen, TEF1
 <br>'''Abbreviations:'''
@@ Line 39: / Line 39: @@
 '''Backbone:'''<br>
-<!--*Backbone name: pSB1C3'/?backbone_name?-->
+*Backbone name: pSB1C3'
 <!--*Resistance: Amp/Cp/Kan/Tet-->
 <!--*Copynumber: low/medium/high-->
@@ Line 50: / Line 50: @@
 '''Enzymatic activity:'''
-<!--none/EC-number ?.?.?.?-->
+EC-number 4.2.3.20
 '''Cytotoxicity:'''<br>
@@ Line 79: / Line 79: @@
 '''Organism:'''<br>
-<!--*Genesequence derived from ''?organism_name?''-->
+*Genesequence derived from ''citrus limon''
 <!--*Codonoptimized for ''?organism_name?''-->
 <!--*Designed for the following Chassis: ''?organism-name?''-->