Difference between revisions of "Part:BBa K864121"

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[[Image:BBa_K864121onplate.JPG|thumbnail|Appearance of pUCori-redq, in pSB4K15Iq and DH5α, on LA plate. ]]
 
<partinfo>BBa_K864121 short</partinfo>
 
<partinfo>BBa_K864121 short</partinfo>
  
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This is a minimal high-copy replication origin coupled to the  constitutive RFP casette <partinfo>BBa_K864502</partinfo>. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pMB1ori-redq".
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This is a minimal high-copy replication origin coupled to the  constitutive RFP cassette <partinfo>BBa_K864502</partinfo>. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-redq".
  
  

Latest revision as of 18:44, 29 September 2012

Appearance of pUCori-redq, in pSB4K15Iq and DH5α, on LA plate.

pMB1 ori - constitutive RFP

This is a minimal high-copy replication origin coupled to the constitutive RFP cassette BBa_K864502. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-redq".


Usage and Biology

Usable as default insert of low and medium copy backbones, especially in lacI positive systems. This is the default insert of the pSB4x15Iq and pSB8x15 series of backbones.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 1434
  • 1000
    COMPATIBLE WITH RFC[1000]