Difference between revisions of "Part:BBa K864680"
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<partinfo>BBa_K864680 short</partinfo>
 
<partinfo>BBa_K864680 short</partinfo>
  
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tetM is a tetracycline resistance gene. (Martin. et al. 1986)
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TetM is a tetracycline resistance gene, encoding a ribosomal protection protein. (Martin. et al. 1986)
  
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We made some characterization of this gene. E coli Top10 could grow on tetracycline (12 µg/ml) with <partinfo>J23110</partinfo>-<partinfo>B0034</partinfo>-tetM in a middle copy backbone.  
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This gene confers tetracycline resistance (at least 12 µg/ml to E coli Top10 when assembled as <partinfo>J23110</partinfo>-<partinfo>B0034</partinfo>-tetM in a middle copy backbone.  
  
 
===Reference===
 
===Reference===

Revision as of 18:20, 29 September 2012

tetM

TetM is a tetracycline resistance gene, encoding a ribosomal protection protein. (Martin. et al. 1986)

This gene confers tetracycline resistance (at least 12 µg/ml to E coli Top10 when assembled as BBa_J23110-BBa_B0034-tetM in a middle copy backbone.

Reference

Nucleic Acids Res. 1986 September 11; 14(17): 7047–7058. PMCID: PMC311716 Nucleotide sequence of the tetM tetracycline resistance determinant of the streptococcal conjugative shuttle transposon Tn1545. P Martin, P Trieu-Cuot, and P Courvalin

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1266