Difference between revisions of "Part:BBa C2003"

 
 
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<partinfo>BBa_C2003 short</partinfo>
 
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This is protein based on Scot Wolfe's Zif268-GCN4(-2) homodimeric zinc finger protein.  It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast).
 
  
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This is protein based on Scot Wolfe's Zif268-GCN4 homodimeric zinc finger protein.  It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast).  Scot optimized the linker between the zinc finger domain and the leucine zipper domain to increase affinity of the homodimer for the 12bp operator.
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_C2003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_C2003 SequenceAndFeatures</partinfo>
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_C2003 parameters</partinfo>
 
<partinfo>BBa_C2003 parameters</partinfo>
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Latest revision as of 19:13, 5 June 2006

Homodimeric zinc finger


This is protein based on Scot Wolfe's Zif268-GCN4 homodimeric zinc finger protein. It is a fusion of fingers 2 and 3 of Zif268 (mouse) and the leucine zipper domain of GCN4 (yeast). Scot optimized the linker between the zinc finger domain and the leucine zipper domain to increase affinity of the homodimer for the 12bp operator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]