Difference between revisions of "Part:BBa K909009"
 
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<partinfo>BBa_K909009 short</partinfo>
 
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[[Image:UVR8_2.png|frameless|160px|right|thumb| UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers.
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[[Image:UVR8_2.png|frameless|200px|right|thumb| UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers. [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)]
 
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UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.  
 
UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.  
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===Usage and Biology===
 
===Usage and Biology===
  
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Differently from other photoreceptors, UVR8 uses tryptophane residues present in the structure, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes at the dimer interface is later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [Christie 2012]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [Heijde 2012].  
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Differently from other photoreceptors, UVR8 uses tryptophane residues present in the dimer interfase, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes and later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [http://www.sciencemag.org/content/335/6075/1492.long (Christie 2012)]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)].  
  
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In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (<partinfo>BBa_K909007</partinfo>)), to use UVR8 as a UV-B sensing domain (<partinfo>BBa_K909008</partinfo>).
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We used UVR8 to create a novel UV-B switch in ''E.coli'', by fusing it with TetR DNA binding domain (<partinfo>BBa_K909008</partinfo>). Additionally we inserted BamHI site after ATG codon for similar protein-protein fusions with this biobrick.
  
 
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Latest revision as of 00:20, 27 September 2012

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana

UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers. [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)]

UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.

Usage and Biology

Differently from other photoreceptors, UVR8 uses tryptophane residues present in the dimer interfase, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes and later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [http://www.sciencemag.org/content/335/6075/1492.long (Christie 2012)]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)].

We used UVR8 to create a novel UV-B switch in E.coli, by fusing it with TetR DNA binding domain (BBa_K909008). Additionally we inserted BamHI site after ATG codon for similar protein-protein fusions with this biobrick.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 76
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 91