Difference between revisions of "Part:BBa K909009"

Revision as of 23:44, 26 September 2012

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana

UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers. [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)]

UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.

Usage and Biology

Differently from other photoreceptors, UVR8 uses tryptophane residues present in the structure, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes at the dimer interface is later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [http://www.sciencemag.org/content/335/6075/1492.long (Christie 2012)]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)].

In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (BBa_K909007)), to use UVR8 as a UV-B sensing domain (BBa_K909008).

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 560
Illegal PstI site found at 1214
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 76
Illegal PstI site found at 560
Illegal PstI site found at 1214
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 560
Illegal PstI site found at 1214
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 560
Illegal PstI site found at 1214
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 91

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K909009 short</partinfo>
-[[Image:UVR8_2.png|frameless|160px|right|thumb| UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers.
+[[Image:UVR8_2.png|frameless|200px|right|thumb| UVR8 as a symmetric homodimer. Upon UV-B exposure the the dimer dissociates into two monomers. [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)]
 ]]
 UVR8 (UV resistance locus 8) is UV-B photoreceptor found in plants as a crucial mediator of plants response to UV-B response. In absence of UV-B the protein occurs as a dimer, which breaks upon UV-B irradiation.
 <!-- Add more about the biology of this part here -->
 ===Usage and Biology===
-Differently from other photoreceptors, UVR8 uses tryptophane residues present in the structure, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes at the dimer interface is later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [Christie 2012]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [Heijde 2012].
+Differently from other photoreceptors, UVR8 uses tryptophane residues present in the structure, rather than external chromophores. Energy from UV-B light is absorbed by these triptophanes at the dimer interface is later used to break dimer cross-salt bridges resulting in the dissociation of the UVR8 monomers [http://www.sciencemag.org/content/335/6075/1492.long (Christie 2012)]. Monomeric UVR8 is then able to initiate downstream reactions that trigger the transcription of proteins needed for the UV-B caused stress response [http://www.cell.com/trends/plant-science/abstract/S1360-1385%2812%2900008-8 (Heijde 2012)].
 In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (<partinfo>BBa_K909007</partinfo>)), to use UVR8 as a UV-B sensing domain (<partinfo>BBa_K909008</partinfo>).