Difference between revisions of "Part:BBa K784015"
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This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br> | This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation. <br> | ||
Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br> | Each fragment was inserted into pSB1C3_MCS plasmid [[Part:BBa_K784023|(BBa_K784023)]], which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome. <br> | ||
| − | More information is available [[http://2012.igem.org/Team:Technion/Project/Phage| here ]]. <br> | + | More information is available [[http://2012.igem.org/Team:Technion/Project/Phage| here]]. <br> |
Fragment 1 contains the phages head proteins, which together with fragment 2 [[Part:BBa_K784016| (BBa_K784016)]], 3 [[Part:BBa_K784017| (Part:BBa_K784017)]] and 4 [[Part:BBa_K784018| (BBa_K784018)]] create the phage envelope. <br> | Fragment 1 contains the phages head proteins, which together with fragment 2 [[Part:BBa_K784016| (BBa_K784016)]], 3 [[Part:BBa_K784017| (Part:BBa_K784017)]] and 4 [[Part:BBa_K784018| (BBa_K784018)]] create the phage envelope. <br> | ||
Revision as of 19:16, 26 September 2012
Phage Lambda fragment 1 - head proteins
This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phages genome.
More information is available here.
Fragment 1 contains the phages head proteins, which together with fragment 2 (BBa_K784016), 3 (Part:BBa_K784017) and 4 (BBa_K784018) create the phage envelope.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2577
Illegal PstI site found at 2841
Illegal PstI site found at 3646
Illegal PstI site found at 3661
Illegal PstI site found at 3877
Illegal PstI site found at 4391
Illegal PstI site found at 4730 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2577
Illegal PstI site found at 2841
Illegal PstI site found at 3646
Illegal PstI site found at 3661
Illegal PstI site found at 3877
Illegal PstI site found at 4391
Illegal PstI site found at 4730 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 436
Illegal BamHI site found at 5526 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2577
Illegal PstI site found at 2841
Illegal PstI site found at 3646
Illegal PstI site found at 3661
Illegal PstI site found at 3877
Illegal PstI site found at 4391
Illegal PstI site found at 4730 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2577
Illegal PstI site found at 2841
Illegal PstI site found at 3646
Illegal PstI site found at 3661
Illegal PstI site found at 3877
Illegal PstI site found at 4391
Illegal PstI site found at 4730
Illegal AgeI site found at 4751
Illegal AgeI site found at 4900
Illegal AgeI site found at 6582 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 2418
Illegal SapI.rc site found at 6510