Difference between revisions of "Part:BBa K823030"
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We used the promtoer P<sub>''cotYZ''</sub> for the regulation of spore crust proteins in ''B. subtilis''. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
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We used the promtoer P<sub>''cotYZ''</sub>, P<sub>''cotV''</sub> and P<sub>''cgeA''</sub> for the regulation of spore crust proteins in ''B. subtilis''. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-''luxABCDE'' ([[Part:BBa_K823025|BBa_K823025]]) and measured the luminescence.
  
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These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P<sub>''cotV''</sub> [[Part:BBa_K823033|BBa_K823033]] with each two clones.
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These are results of a plate reader assay to evaluate promoter activity. Promoters were cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P<sub>''cotV''</sub> [[Part:BBa_K823033|BBa_K823033]] and P<sub>''cgeA''</sub>.
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{| style="color:black;" cellpadding="3" width="70%" cellspacing="0" border="0" align="center" style="text-align:left;"
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|[[File:Promotoren.png|Protein distribution in spore coat of ''Bacillus subtilis''|610px|center]]
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{| style="color:black;" cellpadding="0" width="90%" cellspacing="0" border="0" align="center" style="text-align:left;"
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|style="width: 70%;background-color: #EBFCE4;" |
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<font color="#000000"; size="2"><p align="justify">The result of the measurement of the three promoters P<sub>''cgeA''</sub>, P<sub>''cotV''</sub> and P<sub>''cotYZ''</sub>. The left vertical axis displays the luminescence divided by the OD<sub>600</sub> and the left one shows the OD<sub>600</sub>. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.</p></font>
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[[Image:Activity PcotYZ PcotV.png|thumb|center|400px|These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' ([[Part:BBa_K823033|BBa_K823033]]) with each two clones.]]
 
[[Image:Activity PcotYZ PcotV.png|thumb|center|400px|These are results of a plate reader assay to evaluate promoter activity. P<sub>''cotYZ''</sub> was cloned into [[Part:BBa_K823025|pSBBS3C-''luxABCDE'']] and luminescence was measured. This graph also shows activiy of the related promoter P''cotV'' ([[Part:BBa_K823033|BBa_K823033]]) with each two clones.]]
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This part was used to display fusion proteins on ''B. subtilis'' spores with [[Part:BBa_K823049|BBa_K823049]].
 
This part was used to display fusion proteins on ''B. subtilis'' spores with [[Part:BBa_K823049|BBa_K823049]].
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[[Glowing_spore.png|100px|link=https://parts.igem.org/Part:BBa_K823049]]
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[[File:Glowing_spore.png|100px|link=Part:BBa_K823049]]
  
  

Revision as of 15:10, 26 September 2012

PcotYZ: B. subtilis promoter regulating expression of spore crust proteins

PcotYZ: Promoter of B. subtilis that is involved in spore crust formation. Hence it is activated in late sporulation. PcotYZ regulates the crust protein cotZ and cotY.

CotVWXYZ cluster. (modified after [http://www.ncbi.nlm.nih.gov/pubmed/7519271 Zhang et al., 1994)]


We used the promtoer PcotYZ, PcotV and PcgeA for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

These are results of a plate reader assay to evaluate promoter activity. Promoters were cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033 and PcgeA.

610px

The result of the measurement of the three promoters PcgeA, PcotV and PcotYZ. The left vertical axis displays the luminescence divided by the OD600 and the left one shows the OD600. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.
These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV (BBa_K823033) with each two clones.


This part was used to display fusion proteins on B. subtilis spores with BBa_K823049. Glowing spore.png


See all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]