Difference between revisions of "Part:BBa K929004"
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K929004 short</partinfo>
 
<partinfo>BBa_K929004 short</partinfo>
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{| style="color:black" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K929004 modified_AID+eGFP]
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|-
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! colspan="2"|[[Image:UP12_Part_mod_AIDandeGFP.png|300px]]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_929004 BBa_929004]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_10 RFC 10] and aditional AgeI site
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|-
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|'''Requirement'''
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|pSB1C3<br>
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|-
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|'''Source'''
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|existing parts: BBa_K404316 and K929001
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|-
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|'''Submitted by'''
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|[http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
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|}
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<br>
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[[Image:UP12_Plasmid_modAIDandeGFP.png|left|thumb|400px]]<br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br><br>
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This is a composite part from the existing parts: modified_AID ([https://parts.igem.org/Part:BBa_K929001 BBa_K929001]) and eGFP ([https://parts.igem.org/Part:BBa_K404316 BBa_K404316]). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see [https://parts.igem.org/Part:BBa_K929004:Design Part_Design]. This part was '''not''' used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see [https://parts.igem.org/Part:BBa_K929002 BBa_929002].
 
This is a composite part from the existing parts: modified_AID ([https://parts.igem.org/Part:BBa_K929001 BBa_K929001]) and eGFP ([https://parts.igem.org/Part:BBa_K404316 BBa_K404316]). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see [https://parts.igem.org/Part:BBa_K929004:Design Part_Design]. This part was '''not''' used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see [https://parts.igem.org/Part:BBa_K929002 BBa_929002].

Revision as of 15:44, 24 September 2012

modified AID+eGFP

modified_AID+eGFP
UP12 Part mod AIDandeGFP.png
BioBrick Nr. BBa_929004
RFC standard RFC 10 and aditional AgeI site
Requirement pSB1C3
Source existing parts: BBa_K404316 and K929001
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]


UP12 Plasmid modAIDandeGFP.png






















This is a composite part from the existing parts: modified_AID (BBa_K929001) and eGFP (BBa_K404316). Modified_AID (BBa_929001) is in RFC 10 cloning standard and has an aditional AgeI restriciton site. Therefore it can be fused with RFC 25 parts (C-terminal of modified AID), see Part_Design. This part was not used to express the fusion protein. It is an usefull intermediate that can be combined with differnt promoters and terminators. We combined this part with CMV-promoter and polyA-hGH (poly adenylation signal sequence)--> see BBa_929002.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1296
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 108
    Illegal BsaI.rc site found at 1225
    Illegal SapI site found at 209