Difference between revisions of "Part:BBa K784009"

 
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<partinfo>BBa_K784009 short</partinfo>
 
<partinfo>BBa_K784009 short</partinfo>
  
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This is a construct containing an [[Part:BBa_K784008|MCS followed by a tac promoter]] followed by the [[Part:BBa_K784005|theophylline riboswitch]]. This construct is one of the two fragments used for the [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] of the [[Part:BBa_K784005|theophylline riboswitch]] with different proteins.
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This part has been created by deleting the aptamer and the additional 5' UTR regions of the [[Part:BBa_K784005|theophylline riboswitch]] using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the [[Part:BBa_K784005|theophylline riboswitch]] remains.<br>
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Due to a mutation, this part differs from [[Part:BBa_K784007|BBa_K784007]].
  
 
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Revision as of 14:09, 16 September 2012

Spacer2+RBS+mCherry

This part has been created by deleting the aptamer and the additional 5' UTR regions of the theophylline riboswitch using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the theophylline riboswitch remains.
Due to a mutation, this part differs from BBa_K784007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]