Difference between revisions of "Part:BBa K784008"

Latest revision as of 18:00, 15 September 2012

MCS+tac promoter

The tac promoter is a functional hybrid promoter, derived from the trp and lac promoters. The sequence here is preceded by an MCS as a by product of the restriction cloning steps from a pUC19 plasmid which contained the original sequence. The promoter was followed by the theophylline riboswitch and the complete construct was used for the [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] process with different proteins.
The promoter can be induced with [http://openwetware.org/wiki/IPTG IPTG].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 16
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K784008 short</partinfo>
-The tac promoter is a functional hybrid promoter, derived from the trp and lac promoters. The sequence here is preceded by an MCS as a by product of the restriction cloning steps from a pUC19 plasmid which contained the original sequence. The promoter was followed by the [[Part:BBa_K784005|theophylline riboswitch]] and the complete construct was used for the [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] process with different proteins.
+The tac promoter is a functional hybrid promoter, derived from the trp and lac promoters. The sequence here is preceded by an MCS as a by product of the restriction cloning steps from a pUC19 plasmid which contained the original sequence. The promoter was followed by the [[Part:BBa_K784005|theophylline riboswitch]] and the complete construct was used for the [http://openwetware.org/wiki/Assembly_pcr Assembly pcr] process with different proteins.<br>
+The promoter can be induced with [http://openwetware.org/wiki/IPTG IPTG].
 <!-- Add more about the biology of this part here