Difference between revisions of "Part:BBa K559011"
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Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment.
 
Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment.
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In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad2. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
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In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K559011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K559011 SequenceAndFeatures</partinfo>
  
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===Usage and Biology===
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It has been used to fuse with GFP <partinfo>BBa_E0040</partinfo> to quantitatively control GFP gene expression by providing different chloride concentration. The results can be seen in [[Part:BBa_K559011:Experience]].
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Also, it has been cooperated with <partinfo>BBa_K559010 </partinfo> halorhodopsin complete system for the photo-tunable gene expression platform. <partinfo>BBa_K559010 </partinfo> co-related between light and intracellular chloride ion, with this Pgad takes chloride ions as the intermediate to fine-control gene expression level.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 19:02, 2 November 2011


Pgad - Intracellular Chloride-Sensing Cassette

Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

It has been used to fuse with GFP BBa_E0040 to quantitatively control GFP gene expression by providing different chloride concentration. The results can be seen in Part:BBa_K559011:Experience.

Also, it has been cooperated with BBa_K559010 halorhodopsin complete system for the photo-tunable gene expression platform. BBa_K559010 co-related between light and intracellular chloride ion, with this Pgad takes chloride ions as the intermediate to fine-control gene expression level.