Difference between revisions of "Part:BBa K590051"

Latest revision as of 22:35, 28 September 2011

Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion

This part encodes a direct gene fusion product of the two enzymes, Acyl-ACP Reductase (AAR) and Aldehyde Decarbonylase (ADC), in a linear configuration with a glycine-serine linker for co-localization.

Usage and Biology

We expected this would optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR closer together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to concurrent work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the BBa_K590048 part page.

For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].

Direct fusion of two proteins using a glycine-serine linker.

GC-MS data shows that this configuration did not increase alkane production. For more info, refer to 2011 UW iGEM Team wiki

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 ===Usage and Biology===
-UW iGEM Team has constructed this fusion product to optimize the alkane production developed by the team by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to previous work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the <partinfo>BBa_K590048</partinfo> part page.
+We expected this would optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR closer together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to concurrent work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the <partinfo>BBa_K590048</partinfo> part page.
 For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
 [[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|430px|thumb|Direct fusion of two proteins using a glycine-serine linker.]]
-[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|430px|thumb|GC-MS data shows that this system wasn't successful at optimizing the alkane production. For more info, refer to 2011 UW iGEM Team wiki]]
+[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|430px|thumb|GC-MS data shows that this configuration did not increase alkane production. For more info, refer to 2011 UW iGEM Team wiki]]