Composite
RC-1C3-HC

Part:BBa_K590048

Designed by: Austin Moon, Casey Ager   Group: iGEM11_Washington   (2011-09-15)

AAR-ADC-PSB1C3-High constitutive

This part consists of a Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) direct gene fusion with a linker for co-localization in a PSB1C3 high consititutive vector. 2011 University of Washington Team has designed and constructed this to see if this can increase the alkane product yield by bringing two enzymes closer to each other. For more information, refer to [http://2011.igem.org/Team:Washington 2011 UW iGEM Team's wiki].

More information about the direct fusion construct BBa_K590051 can be found under the part's page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None