Difference between revisions of "Part:BBa K590051"
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<partinfo>BBa_K590051 short</partinfo> | <partinfo>BBa_K590051 short</partinfo> | ||
− | This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase(AAR)] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)], in a linear configuration with a glycine-serine linker for co-localization. | + | This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase (AAR)] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC)], in a linear configuration with a glycine-serine linker for co-localization. |
Revision as of 20:34, 28 September 2011
Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion
This part encodes a direct gene fusion product of the two enzymes, Acyl-ACP Reductase (AAR) and Aldehyde Decarbonylase (ADC), in a linear configuration with a glycine-serine linker for co-localization.
Usage and Biology
2011 UW iGEM Team has constructed this fusion product to optimize the alkane production developed by the team by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to previous work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the BBa_K590048 part page.
For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]