Difference between revisions of "Part:BBa K566003"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i> Aequorea victoria </i>. It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1. It has low absortion and low quantum yield (40%). | + | Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i> Aequorea victoria </i>. It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. |
| + | |||
| + | \Phi = \frac {\textrm{Number\ of\ photons\ emitted}} {\textrm{Number\ of\ photons\ absorbed}} | ||
Revision as of 18:02, 28 September 2011
CFP optimized for E. coli
Cyan fluorescent protein optimized from part BBa_E0022 with preferential codon usage for improved expression in E. coli.
Usage and Biology
Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed.
\Phi = \frac {\textrm{Number\ of\ photons\ emitted}} {\textrm{Number\ of\ photons\ absorbed}}
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
- 1000COMPATIBLE WITH RFC[1000]