Difference between revisions of "Part:BBa K606026"

Latest revision as of 04:14, 22 September 2011

tetO Array

The tetO array composed by a repetition of a tetO sequence, where YFP:tetR (BBa_K606025) can concentrate by binding. The concentration on the array increase the fluorescence sensibility. Origins of the tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2093
Illegal BamHI site found at 95
Illegal BamHI site found at 601
Illegal BamHI site found at 1171
Illegal BamHI site found at 1752
Illegal BamHI site found at 1960
Illegal BamHI site found at 2577
Illegal BamHI site found at 2727
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 117
Illegal BsaI site found at 735
Illegal BsaI site found at 793
Illegal BsaI.rc site found at 428
Illegal SapI site found at 1179
Illegal SapI site found at 3528

Revision as of 18:05, 12 September 2011 (view source) Kyauy (Talk \| contribs) ← Older edit		Latest revision as of 04:14, 22 September 2011 (view source) BaptisteCouly (Talk \| contribs)
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	<partinfo>BBa_K606026 short</partinfo>		<partinfo>BBa_K606026 short</partinfo>

−	The tetO array composed by a ~~10kb~~ tetO sequence, where YFP:tetR can concentrate ~~and~~ increase the fluorescence sensibility. Origins of the tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004~~) (<partinfo>BBa_K606025</partinfo>~~).	+	The tetO array composed by a repetition of a tetO sequence, where YFP:tetR (<partinfo>BBa_K606025</partinfo>) can concentrate by binding. The concentration on the array increase the fluorescence sensibility. Origins of the tetO Array come from François-Xavier Barre, Andrew Wright and Dave Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).