Difference between revisions of "Part:BBa K515102"
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<partinfo>BBa_K515102 short</partinfo>
 
<partinfo>BBa_K515102 short</partinfo>
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<p><b>Description</b></p>
 
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<p>Translational subunit with included optimised insulator and RBS <a href="https://parts.igem.org/Part:BBa_K515002">BBa_K515002</a>, its expression is under the control of the constitutive promoter <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>. Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H). Device contains 15 bp insulator seuence, which ensures tunability of expression through easy switching of promoters. In addition, insulator sequence allows the translation initiation rate (TIR) of the translational subunit to remain the same, when the promoter is replaced.</p>
 
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<b>Compatibility</b>
 
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<p>Device has been tested in <i>E. coli DH5α</i> strain, inserted in vector backbone <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a>. Note that device is only functional in motile strains of <i>E. coli</i>.</p>
 
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===
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<p><b>This BioBrick has been sequence verified.</b>
 
<p><b>This BioBrick has been sequence verified.</b>
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<h2>Background<h2>
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<p><b>Background</b></p>
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<p>Malate responding chemoreceptor originally found in <i>Pseudomonas aeruginosa</i> PA01<sup>[1]</sup> is a codon optimised translational subunit. This subunit <a href="https://parts.igem.org/Part:BBa_K515002">BBa_K515002</a> contains optimised insulator and RBS sequence, its expression is under the control of constitutive promoter <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>. Device is used as an additional chemoreceptor for endogenous chemotaxis mechanism of <i>E. coli</i>. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).</p>
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<p>Malate responding chemoreceptor originally found <i>Pseudomonas aeruginosa</i> PA01 is a codon optimised translational subunit. This subunit <a href="https://parts.igem.org/Part:BBa_K515002">BBa_K515002</a> contains optimised insulator and RBS sequence, its expression is under the control of constitutive promoter <a href="https://parts.igem.org/Part:BBa_J23100">BBa_J23100</a>. Device is used as an additional chemoreceptor to <i>E. coli</i> endogenous chemotaxis pathway. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H). Device contains 15 bp insulator seuence, which ensures tunability of expression through easy switching of promoters. In addition, insulator sequence allows the translation initiation rate (TIR) of the translational subunit to remain the same, when the promoter is replaced.</p>
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<p> Device contains 15 bp insulator seuence, which ensures tunability of expression through easy switching of promoters. In addition, insulator sequence allows the translation initiation rate (TIR) of the translational subunit to remain the same, when the promoter is replaced.</p>
  
 
<b>Compatibility</b>
 
<b>Compatibility</b>
 
<p>Device has been tested in <i>E. coli DH5α</i> strain, inserted in vector backbone <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a>. Note that device is only functional in motile strains of <i>E. coli</i>.</p>
 
<p>Device has been tested in <i>E. coli DH5α</i> strain, inserted in vector backbone <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a>. Note that device is only functional in motile strains of <i>E. coli</i>.</p>
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<h2>References<h2>
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<p>[1] Alvarez-Ortega C and Harwood CS (2007) Identification of malate chemoreceptor in <i>Pseudomonas aeruginosa</i> by screening for chemotaxis defects in an energy taxis-deficient mutant. <i>Applied and Environmental Microbiology</i> <b>73</b> 7793-7795.</p>
 
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Revision as of 22:50, 21 September 2011

J23100 promoter - PA2652 Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 710
    Illegal NgoMIV site found at 812
    Illegal AgeI site found at 118
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1019


This BioBrick has been sequence verified.

Background

Malate responding chemoreceptor originally found in Pseudomonas aeruginosa PA01[1] is a codon optimised translational subunit. This subunit BBa_K515002 contains optimised insulator and RBS sequence, its expression is under the control of constitutive promoter BBa_J23100. Device is used as an additional chemoreceptor for endogenous chemotaxis mechanism of E. coli. Device responds to L (-) Malic acid, linear formula (HO2CCH2CH(OH)CO2H).

Device contains 15 bp insulator seuence, which ensures tunability of expression through easy switching of promoters. In addition, insulator sequence allows the translation initiation rate (TIR) of the translational subunit to remain the same, when the promoter is replaced.

Compatibility

Device has been tested in E. coli DH5α strain, inserted in vector backbone pSB1C3. Note that device is only functional in motile strains of E. coli.

References

[1] Alvarez-Ortega C and Harwood CS (2007) Identification of malate chemoreceptor in Pseudomonas aeruginosa by screening for chemotaxis defects in an energy taxis-deficient mutant. Applied and Environmental Microbiology 73 7793-7795.