Difference between revisions of "Part:BBa K608351"
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<partinfo>BBa_K608351 short</partinfo>
 
<partinfo>BBa_K608351 short</partinfo>
  
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This composite promoter consists of a constitutive promoter ([https://parts.igem.org/Part:BBa_J23114 BBa_J23114]), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter ([https://parts.igem.org/Part:BBa_R0051 BBa_R0051]).
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This is based on part ([https://parts.igem.org/Part:BBa_K098995 BBa_K098995])
  
 
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heat sensitive cI QPI with high promoter, with 6 instead of 8bp between the RBS and cI repressor ATG
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'''Why did we make this part?''': Because sequencing of ([https://parts.igem.org/Part:BBa_K098995 BBa_K098995]) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor CDS.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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This composite promoter consists of a constitutive promoter ([https://parts.igem.org/Part:BBa_J23114 BBa_J23114]), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter ([https://parts.igem.org/Part:BBa_R0051 BBa_R0051]).
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{| style="color:black; width="75%" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align=center
 
{| style="color:black; width="75%" cellpadding="10%" cellpadding="15%" cellspacing="0" border="1" align=center
 
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Revision as of 15:58, 21 September 2011

(correct) temperature sensitive promoter

This is based on part (BBa_K098995)


Why did we make this part?: Because sequencing of (BBa_K098995) revealed the lack of nucleotides between the RBS and start codon of the temperature sensitive λ cI repressor CDS.


Usage and Biology

This composite promoter consists of a constitutive promoter (BBa_J23114), the temperature sensitive λ cI repressor protein which has a cI857 mutation that results in denaturation of the repressor when the temperature is raised from 30°C to 42°C, and a cI regulated promoter based on the λ pR promoter (BBa_R0051).


Freiburg11 Presentation1.jpg

The temperature sensitive promoter functioning at 28-30°C

Freiburg11 Presentation3.jpg The temperature sensitive promoter functioning at 42°C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]