Difference between revisions of "Part:BBa K265008:Experience"

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<I>Allan Crossman (Edinburgh 2011)</I>
 
<I>Allan Crossman (Edinburgh 2011)</I>
 
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Edinburgh successfully fused this part to Enhanced Yellow Fluorescent Protein to produce <partinfo>BBa_K523013</partinfo>. Since the cells fluoresced, the INP did not prevent correct folding.
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Edinburgh can confirm that the sequence of the part itself is as expected. The part comes in RFC12 format and therefore has an unusual prefix and suffix. We found an extra C base in the suffix at the end of the NotI site:
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<pre>
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Expected: GCTAGC GCGGCCG CTGCAG
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Actual:  GCTAGC GCGGCCGC CTGCAG
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</pre>
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Edinburgh successfully fused this part to Enhanced Yellow Fluorescent Protein (with a short linker sequence) to produce <partinfo>BBa_K523013</partinfo>. Since the cells fluoresced, the INP (plus linker) did not prevent correct folding. Furthermore, we found evidence that YFP was localised to the cell membrane.
 
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Latest revision as of 11:22, 18 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K265008

User Reviews

UNIQabfee3ebb4757f6b-partinfo-00000000-QINU

BBa_K265008 Allan Crossman (Edinburgh 2011)

Edinburgh can confirm that the sequence of the part itself is as expected. The part comes in RFC12 format and therefore has an unusual prefix and suffix. We found an extra C base in the suffix at the end of the NotI site: 

Expected: GCTAGC GCGGCCG CTGCAG
Actual:   GCTAGC GCGGCCGC CTGCAG

Edinburgh successfully fused this part to Enhanced Yellow Fluorescent Protein (with a short linker sequence) to produce BBa_K523013. Since the cells fluoresced, the INP (plus linker) did not prevent correct folding. Furthermore, we found evidence that YFP was localised to the cell membrane.

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UNIQabfee3ebb4757f6b-partinfo-00000004-QINU