Difference between revisions of "Part:BBa K523008"

Revision as of 11:38, 8 August 2011

INP for BioSandwich

This is a modified version of BBa_K265008 for use with the BioSandwich protocol of Edinburgh 2011.

BioSandwich assembly requires an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end of the BioBrick. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.

Incidentally, although this part has been made in RFC10 format, the two extra bases should mean that joining it to an RFC23 part would create a viable fusion.

Usage and Biology

The DNA is a codon-optimised version of the N and C terminal domains of Pseudomonas syringae Ice Nucleation Protein. It has been shown that, when INPNC is fused to another protein (the "passenger"), it causes the passenger to be displayed on the outer membrane of E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 409
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol of Edinburgh 2011.
-BioSandwich assembly requires an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
+BioSandwich assembly requires an in-frame BglII site (formed by the last 2 bases of the RFC10 prefix and the first 4 bases at the start of this BioBrick) and an in-frame SpeI site (present in the RFC10 suffix). Because the SpeI site is out of frame, two extra "G" bases have been added at the end of the BioBrick. When combined with the T at the start of the RFC10 suffix, they code for a glycine residue and correct the frameshift.
 Incidentally, although this part has been made in RFC10 format, the two extra bases should mean that joining it to an RFC23 part would create a viable fusion.