Difference between revisions of "Part:BBa K329055"
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This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916 containing each of us one half of the attR lambda recombination site. | This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916 containing each of us one half of the attR lambda recombination site. | ||
| − | When the both enzyme of the transposon,Int and Xis tn916 are expressed (see <partinfo>BBa_K329035</partinfo> and <partinfo>BBa_K329018</partinfo>)., the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome). The excision permit to create a functionnal attR lambda | + | When the both enzyme of the transposon,Int and Xis tn916 are expressed (see <partinfo>BBa_K329035</partinfo> and <partinfo>BBa_K329018</partinfo>)., the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome). The excision permit to create a functionnal attR lambda recombination site. |
| − | + | This mutant provide a high efficiency excision characterized (see experience part). | |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 19:23, 5 November 2010
[KanR + LacZ] in between MUT_MUT[Lambda] Tn916 Arms
This part is composed of a Kanamycine resistance and LacZ cassette between two mutated arms (left and right) of the transposon Tn916 containing each of us one half of the attR lambda recombination site. When the both enzyme of the transposon,Int and Xis tn916 are expressed (see BBa_K329035 and BBa_K329018)., the both arms link together forming a circular DNA sequence excised from the hosting DNA (plasmid or chromosome). The excision permit to create a functionnal attR lambda recombination site. This mutant provide a high efficiency excision characterized (see experience part).
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 363
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 382
Illegal NheI site found at 405
Illegal SpeI site found at 363 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 363
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 363
- 1000COMPATIBLE WITH RFC[1000]