Reporter
HREx9-ymCh

Part:BBa_K5415003:Design

Designed by: Agnese Gabriela Celma   Group: iGEM24_Latvia-Riga   (2024-09-29)
Revision as of 09:20, 30 September 2024 by Gabriela-c (Talk | contribs) (formatting)

Three sets of triple HRE containging oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeCherry:

ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA

GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT

ATGTACGGTAGCAAGGCATACGTCAAGCACCCAGCTGACATTCCAGATTATTTGAAATTATCTTTTCCTGAAGGGTTTAAATGGGAAAGAGTTATGAATTTTGAG

GATGGTGGTGTTGTCACTGTCACACAAGACTCCAGTTTACAAGATGGTGAGTTCATCTACAAGGTTAAATTGAGAGGTACTAACTTTCCATCAGACGGTCCTGTT

ATGCAAAAGAAAACTATGGGTTGGGAAGCTAGCTCCGAAAGAATGTATCCTGAAGATGGTGCTTTGAAGGGTGAAATTAAGCAAAGATTGAAATTGAAAGATGGT

GGTCATTATGATGCAGAAGTTAAGACAACGTATAAAGCGAAAAAACCAGTTCAGCTTCCAGGTGCTTATAATGTTAATATTAAGTTGGACATTACCTCTCATAAT

GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata

ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt

tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat