Reporter
HREx6-yeBF

Part:BBa_K5415002:Design

Designed by: Agnese Gabriela Celma   Group: iGEM24_Latvia-Riga   (2024-09-29)
Revision as of 09:31, 30 September 2024 by Gabriela-c (Talk | contribs) (formatting)

Two sets of triple HRE containing oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoRV CUT:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeBFP2:

ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGAAGGTGAT

GCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGGTTCAGTGCTTTGCCAGA

TATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAAGATGACGGTACATACAAAACAAGAGCT

GAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAACATCCTCGGTCATAAGCTAGAATACAATTTCAATTCT

CATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGACATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAA

AATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACTCAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTG

GAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATATAAGTAG

TAAGTAG

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag

ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc

agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat