Part:BBa_K5143023

plasmid for integrating our composite parts into the Saccharomyces cerevisiae genome

Description

Our project aims to create a fully bio-sourced and biodegradable insect trap. To achieve this, the base of our trap will be made of cellulose produced by a bacterium in a co-culture system. The other organism in this co-culture is Saccharomyces cerevisiae, a yeast that will secrete a yellow chromoprotein and a bioglue (BBa_K5143022). The adhesion of the yellow chromoprotein to the cellulose, which forms the base of our trap, will make it coloured, trapping insects that are specifically attracted to yellow. To allow the secretion and the cellulose binding of the fwYellow, two elements are fused to the fwYellow gene. 1) Upstream of the fwYellow gene : Alpha-factor (BBa_K5143009), a yeast secretion signal allowing the excretion of the fused yellow protein out of the yeast cell. 2) Downstream of the fwYellow gene : Cellulose Binding Domain (BBa_K5143007), allowing the binding of the fused yellow protein to the cellulose. Because yellow can attract many insects, the chromoprotein could easily be changed by cloning methods in order to change the colour of the trap and attract different targets.

Construction

ECRIRE DES TRUCS ICI POUR LA CONSTRUCTION

References

METTRE LES REF ICI

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 688
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 688
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 688
  Illegal BamHI site found at 1233
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 688
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 688
  Illegal NgoMIV site found at 1203
  Illegal AgeI site found at 1242
  
• 1000
  COMPATIBLE WITH RFC[1000]