Part:BBa_K5088675
Tarakate - Test construct GFP
Contents
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 230
Illegal XhoI site found at 1257 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Background
For initial testing of our measurement system we used the GFP from the plant collection in the iGEM distribution kit. The expression was driven by the 35S CaMV promoter, TMV 5'UTR, and 35S 3'UTR. This system is widely used for constitutive overexpression of transgenes in various plant species, including model organisms and economically important crops (1).
Results
Figure 1: SBOL scheme of Tarakate - Test construct GFP [BBa_K5088675].
Figure 2: Successful expression of avGFP in N. benthamiana leaf disk assay using Tarakate - Test construct GFP [BBa_K5088675].
Figure 3: Successful expression of avGFP in protoplasts of Taraxacum officinale using Tarakate - Test construct GFP [BBa_K5088675].
Figure 4: Successful expression of avGFP in roots of T. kok-saghyz using the CDB protocol and Tarakate - Test construct GFP [BBa_K5088675]. A) Brightfield view of a transformed root of T. kok-saghyz B) The same root imaged under UV light.
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