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  • (cur | prev) 13:21, 9 October 2022‎ Zeniazen5 (Talk | contribs)‎ . . (330 bytes) (+330)‎ . . (A production culture was set up using BL21 ''E. coli'' and induced with 0.5 mM IPTG once their ODs reached 0.5. The cultures were left overnight at 30°C for protein production. The cells were then harvested, pelleted and purified using a HisPur Cobalt...)