View source for Part:BBa K3110039


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<partinfo>BBa_K3110039 short</partinfo>

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and weak RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and  lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying  the strength of the promoter and RBS controlling their production.



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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K3110039 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_K3110039 parameters</partinfo>
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===Characterisation===
The construct could not be characterised due to insufficient time.