Part:BBa_K3110039

Weak Promoter Weak RBS lldP+lldR+lldD

This construct has L-Lactate Permease (lldP), Regulator element (lldR) and L-Lactate Dehydrogenase (lldD) under the control of a weak promoter and weak RBS . lldP permits the entry of L-Lactate inside the bacterial cell, lldR has the dual role of an activator and a repressor and lldD cleaves L-Lactate to pyruvate . Our intention of using this part is to have control over the detection threshold of L-Lactate by the lldPRD regulatory region. We have also designed other parts which produce various higher and lower concentrations of lldP, lldR and lldD by varying the strength of the promoter and RBS controlling their production.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 327
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3649
  Illegal AgeI site found at 463
  Illegal AgeI site found at 926
  Illegal AgeI site found at 1364
  Illegal AgeI site found at 1523
  Illegal AgeI site found at 1535
  Illegal AgeI site found at 2281
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3086
  

Characterisation

The construct could not be characterised due to insufficient time.