View source for Part:BBa K4375013


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<partinfo>BBa_K4375013 short</partinfo>

A myc tag is a polypeptide protein tag derived from the c-myc gene, and we added a SpHI restriction site to its N-terminal.


==Usage and Biology==

The Myc-tag is derived from the human c-myc oncogene and corresponds to amino acid residues 410-419 of the C-terminus of human c-Myc protein. The human c-Myc was discovered as a cellular homolog of v-myc oncogenes, which were identified through analyses of avian tumors1,2. C-Myc is a transcription factor.
A Myc-tag can be used to detect expression of recombinant proteins.
First, the Myc-tag should be genetically fused/cloned to a protein of interest (POI). After expression, the Myc-tagged protein can be captured and identified in crude biological samples. Common applications are immunoprecipitation, co-immunoprecipitation, immunofluorescence, ELISA, flow cytometry, protein purification, and Western blotting.


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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_K4375013 SequenceAndFeatures</partinfo>

==References==

„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/ 


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===Functional Parameters===
<partinfo>BBa_K4375013 parameters</partinfo>
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