View source for Device:BBa D0001

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{|
|'''Description'''
|'''Subparts'''
|-
|BBa_D0001 is a receiver device that can be used to sense the level of a cell-cell signaling molecule [[3OC6HSL|3OC<sub>6</sub>HSL]]. A transcription factor, LuxR (''<partinfo>C0062</partinfo>'') that is active in the presence of [[3OC6HSL|3OC<sub>6</sub>HSL]] is controlled by a TetR (''<partinfo>BBa_C0040</partinfo>'') regulated operator (''<partinfo>BBa_R0040</partinfo>'').  ''Device input'' is [[3OC6HSL|3OC<sub>6</sub>HSL]].  ''Device output'' is [[PoPS]] from a LuxR-regulated operator.  This device is known to work in many ''E. coli'' strains at a range of temperatures and in a variety of media types.

|<html><TABLE class='order_row' style='border: none;'border='0' cellpadding='0' cellspacing='0' color='#000000' bgcolor='#FFFFFF'><TR><TD style='font-size:90%; line-height:100%;text-align:center;'>tetR<br>R0040</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>LuxR<br>I0462</TD><TD style='font-size:90%; line-height:100%;text-align:center;'>lux pR<br>R0062</TD><TR class = 'bar_row'><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=187'><img title='BBa_R0040
promoter (tetR, negative)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=2681'><img title='BBa_I0462
luxR Protein Generator' src='https://parts.igem.org/images/partbypart/icon_generator.png' height='25' border=0></A><TD style='border: none;'><A href='/r/parts/partsdb/view.cgi?part_id=192'><img title='BBa_R0062
Promoter (luxR &amp; HSL regulated -- lux pR)' src='https://parts.igem.org/images/partbypart/icon_regulatory.png' height='25' border=0></A></TABLE></html>
|}

'''Performance'''<br>
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{|{{Table}}
!Experiment<sup>1</sup>
!Characteristic<sup>1</sup>
!Value<sup>1</sup>
|-
|rowspan="3"|[[Part:BBa_F2620:Transfer Function|'''Transfer Function''']]
|''Maximum Output''
|6.6 [[PoPS]] cell<sup>-1</sup>
|-
|''Hill coefficient''
|1.6
|-
|[[Switch Point|''Switch Point'']]
|1.5E-9 M [[3OC6HSL|3OC<sub>6</sub>HSL]], exogenous
|-
|[[Part:BBa_F2620:Response time|'''Response time:''']]
|<1 min
|-
|rowspan="2"|[[Part:BBa_F2620:Specificity|'''Input compatibility''']]
|''Strong response to''
|[[3OC6HSL|3OC<sub>6</sub>HSL]], C<sub>6</sub>HSL , C<sub>7</sub>HSL, 3OC<sub>8</sub>HSL, C<sub>8</sub>HSL
|-
|''Weak response to''
|C<sub>4</sub>HSL, C<sub>10</sub>HSL, C<sub>12</sub>HSL
|-
|rowspan="2"|[[Part:BBa_F2620:Stability|'''Stability''']]
|[[Genetic Stability|''Genetic Stability'']]<br>(Low/High Input)
|>92/>56 generations 
|-
|[[Performance Stability|''Performance Stability'']]<br>(Low/High Input)
|>92/>56 generations
|}
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<sup>1</sup>Measured by Ania Labno and Barry Canton 2006-2007
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'''Compatibility'''<br>
[https://parts.igem.org/cgi/partsdb/pgroup.cgi?pgroup=cell ''Chassis:''] Device has been shown to work in ''<partinfo>BBa_V1000</partinfo>'',''<partinfo>BBa_V1001</partinfo>'',''<partinfo>BBa_V1002</partinfo>'', [[Chassis/Cell-Free_Systems/Commercial_E.coli_S30| E.Coli S30 Extract]] [[Chassis/Cell-Free_Systems/Commercial_E.coli_S30/F2620 | (data)]]<br>
[[Plasmid backbones|''Plasmids:'']] Device has been shown to work on ''<partinfo>pSB3k3</partinfo>'' and ''<partinfo>pSB1A3</partinfo>''<br>
[[Part Types|''Devices:'']] Device has been shown to work with ''<partinfo>BBa_E0430</partinfo>'', ''<partinfo>BBa_E0434</partinfo>'', ''<partinfo>BBa_E0240</partinfo>''<br>
Crosstalk with systems containing ''<partinfo>BBa_C0040</partinfo>''.<br>
[[Help:Signalling|''Cell-Cell Signaling Systems:'']] Crosstalk with devices using [[3OC6HSL|3OC<sub>6</sub>HSL]], C6HSL, C7HSL, C8HSL, C10HSL.
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