View source for Template:TypeIIS/Plasmids/Level2

When four Level 1 parts (TUs) are assembled into a Multi-Transcriptional Unit (MTU) into the following plasmid backbones (with <partinfo>BBa_J04455</partinfo>), the MTU will be flanked by BsaI restriction sites and these 4 bp Fusion Sites.

<html>
<center>
<table class="dnaTable">
	<tr>
		<th>Registry Name</th>
		<th>Loop Name</th>
		<th>Fusion Site 5'</th>
		<th>MTU</th>
		<th>Fusion Site 3'</th>
	</tr>
	<tr>
		<td></html><partinfo>pSB3C01</partinfo><html></td>
		<td>pEven1</td>
		<td class="dnaSeq rightAlign">GGAG</td>
		<td class="centerAlign partType" style="background-color: #00B050;">MTU 1</td>
		<td class="dnaSeq">TACT</td>
	</tr>
	<tr>
		<td></html><partinfo>pSB3C02</partinfo><html></td>
		<td>pEven2</td>
		<td class="dnaSeq rightAlign">TACT</td>
		<td class="centerAlign partType" style="background-color: #FFC001;">MTU 2</td>
		<td class="dnaSeq">AATG</td>
	</tr>
	<tr>
		<td></html><partinfo>pSB3C03</partinfo><html></td>
		<td>pEven3</td>
		<td class="dnaSeq rightAlign">AATG</td>
		<td class="centerAlign partType" style="background-color: #4472C4;">MTU 3</td>
		<td class="dnaSeq">GCTT</td>
	</tr>
	<tr>
		<td></html><partinfo>pSB3C04</partinfo><html></td>
		<td>pEven4</td>
		<td class="dnaSeq rightAlign"> GCTT </td>
		<td class="centerAlign partType" style="background-color: #FF0000;">MTU 4</td>
		<td class="dnaSeq">CGCT</td>
	</tr>
</table>
</center>
</html>

'''Note:''' Currently, Registry samples of these plasmid backbones have not been fully sequenced. Only their prefix and suffix along with their insert <partinfo>BBa_J04455</partinfo> have been verified.