View source for Part:BBa M50056


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<partinfo>BBa_M50056 short</partinfo>

We use Escherichia coli as the chassis organism to express the PETase gene and optimize our codon accordingly. This composite part is made of a rhamnose-inducible promoter, an ampicillin resistance marker, an origin of replication, a secretion tag, a strong RBS, and a PETase gene that contains two mutations. All basic parts except mutated PETase come from DNA 2.0. 
Wild type PETase shows the ability to hydrolyze Polyethylene terephthalate (PET), commonly used for plastics. The two mutations of PETase are identified by the 2016 iGEM Tianjin team to improve the catalytic activity of PET independently. The PETase gene here also incorporates 6 his tag at the end. The sequence is optimized for Escherichia coli.

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===Usage and Biology===

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<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_M50056 SequenceAndFeatures</partinfo>


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===Functional Parameters===
<partinfo>BBa_M50056 parameters</partinfo>
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