Part:BBa_M50056
PETase double mutant I208V and R90A full plasmid information
Wild-type PETase shows the ability to hydrolyze Polyethylene terephthalate (PET), commonly used for plastics. We use Escherichia coli as the chassis organism to express a PETase gene that contains two mutations and optimize our codons accordingly. This composite part is made of a rhamnose-inducible promoter, an ampicillin resistance marker, an origin of replication, a secretion tag, a strong RBS, and a PETase gene that contains two mutations. All basic parts except mutated PETase come from DNA 2.0. The two mutations of PETase are identified by the 2016 iGEM Tianjin team to improve the catalytic activity of PET independently. The PETase gene here also incorporates 6 his tag at the end.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 633
Illegal PstI site found at 864 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 633
Illegal PstI site found at 864 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 633
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 633
Illegal PstI site found at 864 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 633
Illegal PstI site found at 864
Illegal NgoMIV site found at 220 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 388
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