View source for Part:BBa J23106

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<partinfo>BBa_J23106 short</partinfo>
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'''[[User:Jasonk|Jasonk]]:I suspect that J23102 is in this well rather than J23106, I'd sequence it before using.'''


===Usage and Biology===

==Sheffield 2016's Characterisation==
Images to follow soon

===Measured strength===
Sheffield 2016 has improved the characterisation of both BBa_J23100 and BBa_J23106. These parts are a strong and medium promoter respectively, that we have used to design our iron detecting device. We have experimentally validated through fluorimetry that there is indeed a significant difference between expression levels of GFP coupled to the strong and medium promoters. Comparative analysis of promoter strengths can be directly interpreted from the data we obtained. This data can be found both on the original part experience pages of BBa_J23100 and BBa_J23106, as well as at 2016.igem.org/Team:Sheffield/Parts. 

Our team has also improved the function of the BBa_J23100 part, by adding an RBS site downstream of the promoter (BBa_K2016005). BBa_J23100 was originally submitted to the Registry by Berkeley 2006.
<html><center>
<img style="width:50%" src="https://static.igem.org/mediawiki/2016/5/59/T--Sheffield--RyhB-graph1.png">
</center>
<p>Fluorescence of JC28 mutants or W3110 wild types transformed with RyhB-GFP constructs under the control of medium (MedGFP) or strong promoters (StrGFP).</p><br/></html>


<span class='h3bb'>Sequence and Features</span>
<partinfo>BBa_J23106 SequenceAndFeatures</partinfo>

===Functional Parameters===

Relative promoter strength estimates (see [http://2009.igem.org/Team:Groningen/Promoters this page] from Groningen 2009):
{|border="1"
!Reference
!Strength
|-
|<partinfo>BBa_J23100</partinfo>
|0.49
|-
|<partinfo>BBa_J23109</partinfo>
|33
|}
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<partinfo>BBa_J23100 parameters</partinfo>

<partinfo>BBa_J23106 parameters</partinfo>
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