Part:pSB1T3

High copy BioBrick assembly plasmid

This is a high copy plasmid for use in assembly of BioBrick® standard biological parts.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2440
  Illegal NheI site found at 1268
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2446
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 2440
  Illegal BamHI site found at 1414
  Illegal XhoI site found at 1033
  Illegal XhoI site found at 2316
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 2440
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 2440
  Plasmid lacks a suffix.
  Illegal XbaI site found at 2455
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 1440
  Illegal NgoMIV site found at 1808
  Illegal NgoMIV site found at 1968
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.