Protein_Domain

Part:BBa_M50002

Designed by: Nicolas Quach   Group: Stanford BIOE44 - S11   (2016-10-24)


mNeonGreen


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Full sequence for the green fluorescent protein mNeonGreen. Codon optimized for E. coli K12.

Usage and Biology

Since their discovery, fluorescent proteins have been used extensively in biology. mNeonGreen is a monomeric green fluorescent protein derived from Branchiostoma lanceolatum. It is the brightest monomeric green fluorescent protein described to date[1]. This protein was used as an expression reporter in BBa_M50003.

References

[1] Shaner, N.C.; Lambert, G.G.; Chammas, A.; Ni, Y.; Cranfill, P.J.; Baird, M.A.; Sell, B.R.; Allen, J.R.; Day, R.N.; Israelsson, M.; Davidson, M.; Wang, J. 2013. A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum. Nature Methods. 10:407-409.

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