Part:BBa_K562007
Salty_PduABTU BMC Components
This is a composite part comprising a constitutive promoter, which is the tatABCD promoter from E. coli K-12, driving production of the PduA and PduB proteins from Salmonella enterica serovar Typhimurium LT2 (identical to part BBa_K562003) AND proteins PduT and PduU. These are all components of the Salmonella bacterial microcompartment (BMC). The PduB and PduU gene product carry C-terminal 6-His affinity/epitope tags. Production of the PduB and PduU proteins has been verified by Western immunoblotting (anti-penta-His) and production of PduA, PduB, PduT and PduU have been confirmed by 35S-Met labelling. The proteins have also been purified from E. coli by immobilised metal affinity chromatography (IMAC) and identified by tryptic peptide mass fingerprinting. The construct is cloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-ABTU in the Sargent Laboratory, Dundee, UK.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1898
Illegal BamHI site found at 2175 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1441
Illegal AgeI site found at 1426
Illegal AgeI site found at 2040 - 1000COMPATIBLE WITH RFC[1000]
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