Regulatory

Part:BBa_K553021

Designed by: Luca Braga, Niels Ntamati   Group: iGEM11_UNITS_Trieste   (2011-09-17)

Mammalian Tra-R inducible element

This part constitutes the core of the TraR binding site (Tra-Box). When combined to have different repeats (1 to 7) and associated to a minimal CMV promoter (CMVmin, BBa_553022), it can be used as a mammalian TraR-inducible gene expression system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 23
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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