RNA
Part:BBa_K5252007
Designed by: Samuel Williams Group: iGEM24_EPFL (2024-09-28)
RDX Riboswitch (clone 4)
RDX-sensitive riboswitch with rapid response between 0 and 4.4 mmol l-1 of RDX (Research Department eXplosive or 1,3,5-Trinitroperhydro-1,3,5-triazine). Above 4.4 mmol l-1 the response saturates. [1]
Acts at the translational level.
References
[1] Eberly JO, Mayo ML, Carr MR, Crocker FH, Indest KJ. Detection of hexahydro-1,3-5-trinitro-1,3,5-triazine (RDX) with a microbial sensor. J Gen Appl Microbiol. 2019 Jul 19;65(3):145-150. doi: 10.2323/jgam.2018.08.001. Epub 2019 Jan 29. PMID: 30700648.
https://pubmed.ncbi.nlm.nih.gov/30700648/
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 57
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 57
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 57
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 57
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |