Regulatory

Part:BBa_K523005

Designed by: Damian Barnard   Group: iGEM11_Edinburgh   (2011-07-29)

Plac-RBS-LacZ-RBS

Plac promoter (with RBS) followed by LacZ reporter followed by another RBS.

Usage and Biology

This is intended to be used as a promoter and RBS; i.e. it can be added upstream of a coding sequence to cause that sequence to become expressed.

The LacZ reporter will cause lab strains with the correct genotype to turn blue in the presence of Xgal+IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None