Part:BBa_K5090010
BBa_J61100 + miRNA-326 Target Site (BBa_K5090006)
Description
This part contains the first 12 nucleotides of BBa_J61100 and 18 nucleotides of miRNA-326 (in between the middle and end of the 21-nucleotide sequence), in addition to the start codon. The rationale for which nucleotides were of each original part was based on maximizing the functionality of the mixed part while staying within the limits of bacterial gene expression. As such, we retained the original 12 nucleotides of BBa_J61100 to retain the ability of the ribosome to bind to the site as much as possible, while adding 18 nucleotides of miRNA-326 to ensure Argonaute 2, when expressed alongside a gene under this RBS, will still be able to bind to the sequence.
This part was not characterized at the time of writing due to time constraints.
Usage and Biology
This part, if successfully designed, would allow for the gene which encourages RBS binding to be regulated by miRNA and Argonaute2. However, it is not the most likely that this would work due to much of the RBS and three nucleotides of the microRNA target site being missing, potentially inhibiting either individual part’s functionality, even though those decisions were made based on constraints with bacterial gene expression. This part was uploaded for future iGEM teams to potentially test and confirm whether or not the constraints prevent the part from performing its function.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
| None |