Coding

Part:BBa_K4907037

Designed by: Tantan Cheng   Group: iGEM23_XMU-China   (2023-09-14)


rfp

Biology

This part is a monomeric far-red fluorescent protein derived from Entacmaea quadricolor, which has excitation and emission wavelengths at 588 and 633 nm respectively. It is a commonly used reporter for expressing and tracing.

Usage and design

In our co-culture system, we use this part as a growth state indicator for E. coli Nissle 1917. We opted to use pET-28a(+) as the vector of rfp and then transformed it into the bacteria. This part also served as a reporter of the characterization for a range of VSW-3 RNAP expression systems: BBa_K4907108, BBa_K4907110, BBa_K4907111, BBa_K4907113, as well as our "AND GATE" based on the VSW-3 RNAP.
Please click the composite part directly for more detailed information.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 427
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 549
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None