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Part:BBa_K4813011

Designed by: Chong Tsz Ting, Jason   Group: iGEM23_HongKong-JSS   (2023-10-08)


20-bp 3' overlapping sequence for pET28a(+)-FGF2 EcoRI restriction site

In our project, we used this 3' overlapping sequence for NEBuilder HiFi assembly to clone our constructs into pET28a(+)-FGF2 vectors. This particular sequence consists of 20 base pairs, which is the recommended length suggested by the NEBuilder HiFi assembly kit manufacturer. By employing this 3' overlapping sequence, along with a 5' overlapping sequence (BBa_K4813010) , we successfully assembled our four verified constructs (K , K ) using the NEBuilder HiFi assembly method.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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