Plasmid

Part:BBa_K4761420

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


pTarget-pheA1

SgRNA(pheA)1 expression plasmid for pheA knockout. Created based on sgRNA(pheA)1 (BBa_K4761020) and pTarget (BBa_K4761107) using overlapping PCR for guide RNA to function in CRISPR-Cas9 double plasmid system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2064
    Illegal NheI site found at 1932
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2064
    Illegal BglII site found at 2094
    Illegal BamHI site found at 1920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2064
    Illegal XbaI site found at 2070
    Illegal SpeI site found at 1955
    Illegal PstI site found at 2082
    Illegal NgoMIV site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


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