Coding
Part:BBa_K4761002
Designed by: Pinhan Pan Group: iGEM23_HiZJU-China (2023-09-27)
UGT85A1
Coding for a UDP-glycosyltransferase found in Schizonepeta tenuifolia. Generates rhodioloside from UDP-glucose and tyrosol. This UDP-glycosyltransferase is found to be the most suitable one to form rhodioloside.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterizaion
In 2023 HiZJU-China, UGT85A1 gene is used for generating salidroside using UDP-glucose and tyrosol. It is proven to make use of tyrosol produced by ARO10-adh6(BBa_K4761200) and generate salidroside with a stable yield(over 7mg/L and 12mg/L with and without pgm-galU(BBa_K4761201), in 1/50 inoculated M9 broth).
[edit]
Categories
Parameters
None |