Part:BBa_K4727004
M13 Gene3 N2 deletion
The design of this part was based on the fundamental work of DA Marvin[1], which suggested that mutants lacking the N2 domain og Gp3 protein of M13 bacteriphage can infect bacteria without pilus with a 100-fold higher efficiency[1], as N2 masks the interaction of N1 with the co-receptor TolA. Building on this, the idea was to design a gene with a deleted N2 domain, drawing from the findings of Nilsson et al.[2], who precisely delineated the amino acid regions corresponding to individual domains. Therefore, an alternative gene was designed for tropism that could ideally enable E. coli infection in the absence of a pilus, thus extending the tropism. This gene, named was obtained by in silico deletion of the region between residues 87 and 256, corresponding to the N2 domain[2].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 205
References
[1] Marvin, D. Filamentous phage structure, infection and assembly. Curr. Opin. Struct. Biol. 8, 150–158 (1998). [2] Nilsson, N., Malmborg, A.-C. & Borrebaeck, C. A. K. The Phage Infection Process: a Functional Role for the Distal Linker Region of Bacteriophage Protein 3. J. Virol. 74, 4229–4235 (2000)
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