Coding

Part:BBa_K4695041

Designed by: Maciej Małolepszy   Group: iGEM23_IFB-Gdansk   (2023-10-01)


docscaB

Coding sequence for dockerin domain (DocScaB) from the cellulosome of Acetivibrio cellulolyticus.

Design Notes

FLANKED 5' fusion site: TTCG, 3' fusion site: CGGC.
Source sequence was optimized and checked for BsaI and SapI restriction sites using: http://genomes.urv.es/OPTIMIZER/

This part has no START codon at the 5' end of the sequence and no codon STOP at the 3' end because it was designed as a part of a fusion protein with interchangable domains. If you want to express only this coding sequence, please add the START and STOP codons in your design.

Source

Organism: Acetivibrio cellulolyticus
Structure: https://www.rcsb.org/structure/4UYQ

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 91
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 91
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 91
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 91
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None